2BRQ
Crystal structure of the filamin A repeat 21 complexed with the integrin beta7 cytoplasmic tail peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-17 |
Detector | MARRESEARCH MAR165 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.970, 60.000, 51.570 |
Unit cell angles | 90.00, 110.17, 90.00 |
Refinement procedure
Resolution | 37.680 - 2.100 |
R-factor | 0.197 |
Rwork | 0.192 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v05 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.465 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.680 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | 0.050 |
Number of reflections | 13748 | |
<I/σ(I)> | 17.6 | 5 |
Completeness [%] | 96.9 | 96.1 |
Redundancy | 5.2 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 1.26 M SODIUM CITRATE, 0.1 M CITRIC ACID PH 5.5 |