2BME
high resolution structure of GppNHp-bound human Rab4a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-23 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 |
| Unit cell lengths | 99.790, 99.790, 95.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.540 - 1.570 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1huq |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.821 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.750 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.250 |
| Number of reflections | 128819 | |
| <I/σ(I)> | 20.9 | 6.7 |
| Completeness [%] | 99.5 | 97.4 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 8% (W/V) PEG 4000, 100MM SODIUM ACETATE PH 4.0 HANGING DROP 25 DEGREE CELSIUS |
