2BIJ

Crystal structure of the human protein tyrosine phosphatase PTPN5 (STEP, striatum enriched enriched Phosphatase)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-12-02
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 21 21 21
Unit cell lengths	51.808, 64.318, 101.077
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.230 - 2.050
R-factor	0.215
Rwork	0.212
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jln
RMSD bond length	0.015
RMSD bond angle	1.484
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.740	2.150
High resolution limit [Å]	2.050	2.050
Rmerge	0.090	0.370
Number of reflections	20825
<I/σ(I)>	11.14	3.05
Completeness [%]	95.0	97
Redundancy	4.7	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				PROTEIN SOLUTION: 10 MG/ML PTPN5, 50 MM HEPES PH 7.5, 200 MM NACL, 10 MM DTT PRECIPITANT: 25% PEG3350, 0.2 M LI2SO4, 100 MM BIS-TR-S PH 5.5