2BIF
6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE H256A MUTANT WITH F6P IN PHOSPHATASE ACTIVE SITE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1995-03-15 |
Detector | RIGAKU |
Spacegroup name | P 1 |
Unit cell lengths | 61.740, 73.510, 76.700 |
Unit cell angles | 116.90, 99.31, 105.20 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bif |
RMSD bond length | 0.012 |
RMSD bond angle | 23.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.052 * | |
Number of reflections | 37144 * | |
<I/σ(I)> | 21.6 | 3.7 |
Completeness [%] | 95.1 * | 60.6 |
Redundancy | 2.16 * | 1.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 4 * | 90MM SUCCINATE, PH 6.0, 17% PEG4000, 1% B-OCTYLGLUCOSIDE, 10% GLYCEROL, pH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
10 | 1 | drop | EDTA | 0.040 (mM) | |
11 | 1 | reservoir | succinate | 60-90 (mM) | |
12 | 1 | reservoir | glycerol | 10 (%) | |
13 | 1 | reservoir | 10 (mM) | ||
14 | 1 | reservoir | PEG4000 | 17 (%) | |
2 | 1 | drop | Tris-PO4 | 50 (mM) | |
3 | 1 | drop | glycerol | 5 (%) | |
4 | 1 | drop | EGTA | 0.040 (mM) | |
5 | 1 | drop | dithiothreitol | 0.800 (mM) | |
6 | 1 | drop | PEG300 | 0.4 (%) | |
7 | 1 | drop | Fru-6-P | 1.7 (mM) | |
8 | 1 | drop | AMP-PNP | 1.7 (mM) | |
9 | 1 | drop | beta-octylglucoside | 1 (%) |