2BIF
6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE H256A MUTANT WITH F6P IN PHOSPHATASE ACTIVE SITE
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | PROTEIN (6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE) | polymer | 469 | 54001.5 | 2 | UniProt (P25114) Pfam (PF01591) Pfam (PF00300) | Rattus norvegicus (Norway rat) | |
| 2 | C, E, J (A, B) | octyl beta-D-glucopyranoside | non-polymer | 292.4 | 3 | Chemie (BOG) | |||
| 3 | D, K (A, B) | 6-O-phosphono-beta-D-fructofuranose | non-polymer | 260.1 | 2 | Chemie (F6P) | |||
| 4 | F (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | G, L, M (A, B) | PHOSPHATE ION | non-polymer | 95.0 | 3 | Chemie (PO4) | |||
| 6 | H (A) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) | |||
| 7 | I, N (A, B) | SUCCINIC ACID | non-polymer | 118.1 | 2 | Chemie (SIN) | |||
| 8 | O, P (A, B) | water | water | 18.0 | 275 | Chemie (HOH) |
Sequence modifications
A, B: 0 - 468 (UniProt: P25114)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | SEE REMARK 999 |
| Phe 15 | Trp 15 | engineered mutation |
| Phe 64 | Trp 64 | engineered mutation |
| Ala 256 | His 256 | engineered mutation |
| Phe 299 | Trp 299 | engineered mutation |
| Phe 320 | Trp 320 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 108003.0 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 2449.0 | |
| All* | Total formula weight | 110452.0 |
