2BCG

Structure of doubly prenylated Ypt1:GDI complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 1 21 1
Unit cell lengths	47.660, 120.230, 60.550
Unit cell angles	90.00, 90.67, 90.00

Refinement procedure

Resolution	19.920 - 1.480
R-factor	0.16486
Rwork	0.163
R-free	0.19283
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ukv
RMSD bond length	0.013
RMSD bond angle	1.453
Data scaling software	XDS
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.920	1.490
High resolution limit [Å]	1.480	1.480
Number of reflections	109178
<I/σ(I)>	11.32
Completeness [%]	96.5	75.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	293	14% PEG MME 2000, 100 mM Tris-Acetate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K