2BBT
Human deltaF508 NBD1 with two solublizing mutations.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Collection date | 2005-03-11 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.405, 65.686, 132.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.700 - 2.300 |
| R-factor | 0.235 |
| Rwork | 0.231 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xmj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.029 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 63.700 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 23718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 280 | 35% PEG4000, 0.2M LiCl, 50mM Tris pH 7.5, 3mM ATP, 5mM MgCl2, 2mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
