2BBR
Crystal Structure of MC159 Reveals Molecular Mechanism of DISC Assembly and vFLIP Inhibition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.979, 62.867, 75.806 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.200 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bbz |
RMSD bond length | 0.004 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 52241 | |
Completeness [%] | 99.6 | 97.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 1.0 - 1.2 M sodium acetate, 0.1 M imidazole , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |