2B8K

12-subunit RNA Polymerase II

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-05
Detector	ADSC QUANTUM 315
Wavelength(s)	1.279, 1.282, 1.283, 1.276
Spacegroup name	C 2 2 21
Unit cell lengths	220.690, 394.330, 281.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	158.114 - 4.150
R-factor	0.387
Structure solution method	MAD
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES

Data quality characteristics

	Overall
Low resolution limit [Å]	158.114
High resolution limit [Å]	4.150
Number of reflections	77251
Completeness [%]	83.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP