2B4Y
Crystal Structure of Human Sirtuin homolog 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.574, 58.756, 104.965 |
| Unit cell angles | 93.50, 92.75, 94.98 |
Refinement procedure
| Resolution | 40.300 - 1.900 |
| R-factor | 0.18527 |
| Rwork | 0.183 |
| R-free | 0.23604 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ici |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.822 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.300 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 79819 | |
| Completeness [%] | 96.1 | 85.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 273 | 18% PEG 4000, 0.1M Hepes, pH 7.5, 10% Isopropanol, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
