2B1Z
Human estrogen receptor alpha ligand-binding domain in complex with 17methyl-17alpha-dihydroequilenin and a glucoc interacting protein 1 NR box II peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2005-08-09 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.923, 83.799, 58.523 |
| Unit cell angles | 90.00, 108.94, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.780 |
| R-factor | 0.20577 |
| Rwork | 0.204 |
| R-free | 0.23779 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zky |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.951 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.840 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.051 | 0.402 |
| Number of reflections | 48202 | |
| <I/σ(I)> | 21.514 | 3.139 |
| Completeness [%] | 98.6 | 99.1 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | pH 8.50 |
