2AZX
Charged and uncharged tRNAs adopt distinct conformations when complexed with human tryptophanyl-tRNA synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9783 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 114.058, 132.621, 246.633 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.211 |
| Rwork | 0.205 |
| R-free | 0.25200 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.058 | |
| Number of reflections | 87100 | |
| <I/σ(I)> | 22.9 | |
| Completeness [%] | 97.1 | 76.3 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 279 | Ammonium sulfate, Hepes, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 279K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium sulfate | ||
| 2 | 1 | 1 | Hepes | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | Ammonium sulfate | ||
| 5 | 1 | 2 | H2O |
