2AUS
Crystal structure of the archaeal box H/ACA sRNP Nop10-Cbf5 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9686 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 136.430, 136.820, 59.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.225 |
Rwork | 0.224 |
R-free | 0.25200 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.491 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 65534 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | potassium phosphate, sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | potassium phosphate, sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |