2AUG

Crystal structure of the Grb14 SH2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2004-11-15
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 31 2 1
Unit cell lengths	49.118, 49.118, 184.087
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.840 - 2.300
Rwork	0.199
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nrv
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.840	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.073	0.166
Number of reflections	11856
<I/σ(I)>	14.2	14.2
Completeness [%]	99.7	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		pH 7.5