2ASS
Crystal structure of the Skp1-Skp2-Cks1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2004-03-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.04187 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 149.332, 149.332, 99.916 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.940 - 3.000 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fqv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.099 | 0.413 |
| Number of reflections | 25854 | |
| <I/σ(I)> | 10 | 2.8 |
| Completeness [%] | 98.2 | 98.5 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 277 | BTP, sodium/potassium phosphate, benzamidine, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
