2AS8
Crystal structure of mature and fully active Der p 1 allergen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.006768 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.120, 78.430, 83.774 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.600 - 1.950 |
| R-factor | 0.18819 |
| Rwork | 0.185 |
| R-free | 0.25171 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 7PCK |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.999 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.117 | 0.574 |
| Number of reflections | 42045 | |
| <I/σ(I)> | 3 | |
| Completeness [%] | 98.3 | 95.2 |
| Redundancy | 34 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Low salt | 4.5 | 277 | 25mM sodium acetate, pH 4.5, Low salt, temperature 277K |
