2ARP
Activin A in complex with Fs12 fragment of follistatin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-23 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 79.297, 94.502, 44.873 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.800 - 2.000 |
R-factor | 0.20189 |
Rwork | 0.202 |
R-free | 0.25404 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries: 1s4u 1lr9 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.710 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.800 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.427 | |
Number of reflections | 23201 | |
<I/σ(I)> | 23.9 | 2.67 |
Completeness [%] | 98.5 | 98.1 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG-MME 2000, Nickel chloride, Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |