2AQZ
Crystal structure of FGF-1, S17T/N18T/G19 deletion mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2005-08-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.940, 95.129, 108.165 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 1.850 |
| Rwork | 0.196 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.990 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.067 | 0.199 |
| Number of reflections | 31617 | |
| <I/σ(I)> | 55.05 | 7.42 |
| Completeness [%] | 94.2 | 97.1 |
| Redundancy | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | Sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
