2ANN
Crystal structure (I) of Nova-1 KH1/KH2 domain tandem with 25 nt RNA hairpin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-27 |
Detector | APS-1 |
Wavelength(s) | 1.0080 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 155.304, 37.147, 34.825 |
Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.196 |
Rwork | 0.193 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Nova-2 KH3 domain from PDB ENTRY 1EC6 and idealised 5 bp A-RNA |
RMSD bond length | 0.011 |
RMSD bond angle | 1.335 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.068 | 0.444 |
Number of reflections | 9118 | |
Completeness [%] | 99.7 | 97.9 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 293 | PEG 4000, isopropanole, sodium citrate, potassium chloride, magnesium chloride, pH 5.6, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
10 | 1 | 2 | magnesium chloride | ||
2 | 1 | 1 | isopropanole | ||
3 | 1 | 1 | sodium citrate | ||
4 | 1 | 1 | potassium chloride | ||
5 | 1 | 1 | magnesium chloride | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | PEG 4000 | ||
8 | 1 | 2 | sodium citrate | ||
9 | 1 | 2 | potassium chloride |