2AIX

X-ray structure of the GLUR2 ligand-binding core (S1S2J) in complex with (s)-thio-atpa at 2.2 a resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-10-14
Detector	MARRESEARCH
Spacegroup name	P 41 2 2
Unit cell lengths	47.875, 47.875, 240.664
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.170
R-factor	0.198
Rwork	0.198
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1M5B.
RMSD bond length	0.005
RMSD bond angle	1.220
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.250
High resolution limit [Å]	2.170	2.170
Rmerge	0.067	0.228
Number of reflections	15624
<I/σ(I)>	24.6	7.6
Completeness [%]	98.3	93.4
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	279	SODIUM CHLORIDE, CACODYLATE, PEG 8000, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 279K