2AF9
Crystal Structure analysis of GM2-Activator protein complexed with phosphatidylcholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2003-08-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97943 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.120, 41.980, 114.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.500 - 2.000 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pub |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.040 | 0.292 |
| Number of reflections | 13297 | |
| <I/σ(I)> | 34 | 3.1 |
| Completeness [%] | 92.6 | 65 |
| Redundancy | 5.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 278 | Peg 4000, iso-propanol, Hepes, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
