2ABI
Crystal structure of the human mineralocorticoid receptor ligand-binding domain bound to deoxycorticosterone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 69 |
| Detector technology | CCD |
| Collection date | 2004-04-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979870 |
| Spacegroup name | P 31 |
| Unit cell lengths | 119.770, 119.770, 41.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.200 - 2.330 |
| R-factor | 0.2074 |
| Rwork | 0.207 |
| R-free | 0.26390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1Y9R |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BEAST |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 2.070 |
| High resolution limit [Å] | 2.330 | 1.960 |
| Rmerge | 0.147 | 0.662 |
| Number of reflections | 27439 | |
| <I/σ(I)> | 10.52 | 1.7 |
| Completeness [%] | 87.5 | 86.4 |
| Redundancy | 3.192 | 2.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 297 | PEG4000, HEPES, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
