2ABI
Crystal structure of the human mineralocorticoid receptor ligand-binding domain bound to deoxycorticosterone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 69 |
Detector technology | CCD |
Collection date | 2004-04-08 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979870 |
Spacegroup name | P 31 |
Unit cell lengths | 119.770, 119.770, 41.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.200 - 2.330 |
R-factor | 0.2074 |
Rwork | 0.207 |
R-free | 0.26390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 1Y9R |
RMSD bond length | 0.013 |
RMSD bond angle | 1.200 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | BEAST |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.200 | 2.070 |
High resolution limit [Å] | 2.330 | 1.960 |
Rmerge | 0.147 | 0.662 |
Number of reflections | 27439 | |
<I/σ(I)> | 10.52 | 1.7 |
Completeness [%] | 87.5 | 86.4 |
Redundancy | 3.192 | 2.03 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 297 | PEG4000, HEPES, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 297K |