2A1X
Human phytanoyl-coa 2-hydroxylase in complex with iron and 2-oxoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9800 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 67.929, 86.677, 97.541 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.440 - 2.500 |
| R-factor | 0.20772 |
| Rwork | 0.204 |
| R-free | 0.27428 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.360 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.440 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.119 | |
| Number of reflections | 9583 | |
| <I/σ(I)> | 13.5 | 2 |
| Completeness [%] | 94.2 | 97.2 |
| Redundancy | 5.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | 20% PEG 3350, 0.3 M tri-ammonium citrate, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
