2A1U
Crystal structure of the human ETF E165betaA mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-05 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8123 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.086, 62.869, 174.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.740 - 2.110 |
R-factor | 0.1826 |
Rwork | 0.179 |
R-free | 0.24695 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1efv |
RMSD bond length | 0.013 |
RMSD bond angle | 1.424 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.084 | 0.266 |
Number of reflections | 28046 | |
<I/σ(I)> | 14.4 | 3.4 |
Completeness [%] | 91.0 | 62.2 |
Redundancy | 4.6 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG2000MME, 0.1 magnesium sulphate, 0.1M cacodylic acid, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |