2ZVM
Crystal structure of PCNA in complex with DNA polymerase iota fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.620, 68.820, 90.180 |
| Unit cell angles | 90.00, 95.05, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.300 |
| R-factor | 0.19627 |
| Rwork | 0.193 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vym |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.790 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.067 | 0.338 |
| Number of reflections | 44815 | |
| <I/σ(I)> | 13.5 | 3.2 |
| Completeness [%] | 98.0 | 86.4 |
| Redundancy | 4.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP VAPOR DIFFUSION | 6.4 | 293 | pH6.4, HANGING DROP VAPOR DIFFUSION, temperature 293K |
