2ZO9
Malonate-bound structure of the glycerophosphodiesterase from Enterobacter aerogenes (GpdQ) and characterization of the native Fe2+ metal ion preference
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2947 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 164.159, 164.159, 164.159 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.030 - 2.200 |
| R-factor | 0.17059 |
| Rwork | 0.170 |
| R-free | 0.18906 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dxn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.602 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 73371 | |
| <I/σ(I)> | 20.9 | 1.6 |
| Completeness [%] | 98.2 | 94.1 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | pH 7, VAPOR DIFFUSION, temperature 277K |
