2ZME
Integrated structural and functional model of the human ESCRT-II complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-06-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 81.480, 81.480, 226.244 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.740 - 2.900 |
| R-factor | 0.21928 |
| Rwork | 0.214 |
| R-free | 0.31277 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cuq |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.728 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 19408 | |
| <I/σ(I)> | 41.3 | 3.25 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 300 | 40% PEG300, 0.1M Tris-HCl, pH8.5, VAPOR DIFFUSION, SITTING DROP |
