2ZA5

Crystal Structure of human tryptase with potent non-peptide inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00
Spacegroup name	P 41
Unit cell lengths	82.678, 82.678, 170.284
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.915 - 2.300
R-factor	0.184
Rwork	0.181
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a0l
RMSD bond length	0.009
RMSD bond angle	1.264
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.300
Number of reflections	51904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.1		pH 6.1, VAPOR DIFFUSION, SITTING DROP