2Z5S
Molecular basis for the inhibition of p53 by Mdmx
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2007-02-18 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.520, 30.440, 100.850 |
| Unit cell angles | 90.00, 102.47, 90.00 |
Refinement procedure
| Resolution | 19.860 - 2.300 |
| R-factor | 0.25065 |
| Rwork | 0.247 |
| R-free | 0.31020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t4f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.426 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.135 | 0.300 |
| Number of reflections | 21546 | |
| <I/σ(I)> | 4 | |
| Completeness [%] | 91.6 | 82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 280 | 30% PEG 300, 0.1M MES pH6.5, VAPOR DIFFUSION, SITTING DROP, temperature 280K |
