2YDV
Thermostabilised HUMAN A2a Receptor with NECA bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.936, 99.551, 79.655 |
| Unit cell angles | 90.00, 93.31, 90.00 |
Refinement procedure
| Resolution | 79.520 - 2.600 |
| R-factor | 0.23413 |
| Rwork | 0.233 |
| R-free | 0.25809 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eml |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.005 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0100) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.410 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.080 | 0.570 |
| Number of reflections | 17471 | |
| <I/σ(I)> | 8.5 | 1.7 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 3.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.4 | 0.05 M ADA NAOH, PH 6.4, 23.6% V/V PEG 400, 4% V/V 2-PROPANOL. |
