2Y9Q

Crystal structure of human ERK2 complexed with a MAPK docking peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-07-25
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 21 21 21
Unit cell lengths	65.360, 65.880, 95.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.856 - 1.550
R-factor	0.1557
Rwork	0.155
R-free	0.17680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gph
RMSD bond length	0.009
RMSD bond angle	1.327
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.860	1.630
High resolution limit [Å]	1.550	1.550
Rmerge	0.070	0.650
Number of reflections	59403
<I/σ(I)>	15.82	1.92
Completeness [%]	98.6	91.4
Redundancy	5.32	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		12% PEG 20000, 100 MM MIB BUFFER PH 6.5 .