2Y34

S-nitrosylated PHD2 (NO exposed) in complex with Fe(II) and UN9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-13
Detector	ADSC CCD
Spacegroup name	P 63
Unit cell lengths	110.302, 110.302, 40.378
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.580 - 2.010
R-factor	0.221
Rwork	0.221
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g19
RMSD bond length	0.011
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.580	2.080
High resolution limit [Å]	2.010	2.010
Rmerge	0.070	0.570
Number of reflections	18814
<I/σ(I)>	24.42	2.07
Completeness [%]	99.0	94.7
Redundancy	2.9	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5		1.6M AMMONIUM SULFATE, 4% DIOXANE, 0.1M MES PH6.5, VAPOR DIFFUSION, HANGING DROP, PRE-GROWN CRYSTALS WERE EXPOSED TO NITRIC OXIDE SATURATED SOLUTION (1 ML, DENSITY 1.34 G/L) FOR AN HOUR.