2Y1N
Structure of c-Cbl-ZAP-70 peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 93.570, 93.570, 189.599 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.423 - 1.999 |
| R-factor | 0.159 |
| Rwork | 0.158 |
| R-free | 0.18040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cbl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.142 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.520 |
| Number of reflections | 63238 | |
| <I/σ(I)> | 30.3 | 2.5 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18-20% (W/V) PEG 3350, 0.2 M AMMONIUM FORMATE |
