2XZO
Upf1 helicase - RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-03-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.066, 101.896, 112.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.184 - 2.395 |
| R-factor | 0.1947 |
| Rwork | 0.193 |
| R-free | 0.23290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wjv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.024 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.180 | 2.520 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.590 |
| Number of reflections | 35441 | |
| <I/σ(I)> | 14.5 | 3.3 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 6.2 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 100 MM SODIUM ACETATE PH 4.6, 20 MM CACL2, 15% MPD |
