2XXZ

Crystal structure of the human JMJD3 jumonji domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-30
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	56.757, 71.431, 159.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.160 - 1.800
R-factor	0.18486
Rwork	0.183
R-free	0.21578
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.013
RMSD bond angle	1.351
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.160	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.100	0.960
Number of reflections	61104
<I/σ(I)>	8.5	2
Completeness [%]	99.9	100
Redundancy	4.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1