2XSX
Crystal structure of human beta enolase ENOB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-31 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.878, 105.531, 106.091 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.390 - 1.700 |
| R-factor | 0.15463 |
| Rwork | 0.153 |
| R-free | 0.18905 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1TE6 2akm 2akz 2PSN AND 3B97 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.525 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.490 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.720 |
| Number of reflections | 114102 | |
| <I/σ(I)> | 15.7 | 3.1 |
| Completeness [%] | 96.4 | 94.6 |
| Redundancy | 8.7 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M NA_ACETATE, 25% PEG_8000, 0.1M CACODYLATE PH 7 |
