2XRL
Tet-repressor class D T103A with doxycycline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-29 |
| Detector | RIGAKU-MSC |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 67.888, 67.888, 180.438 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.870 - 1.850 |
| R-factor | 0.238 |
| Rwork | 0.236 |
| R-free | 0.27908 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o7o |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.854 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.540 |
| Number of reflections | 19733 | |
| <I/σ(I)> | 13 | 1.7 |
| Completeness [%] | 98.4 | 88.6 |
| Redundancy | 9.17 | 3.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | HANGING DROP VAPOR DIFFUSION WITH 2 UL (14 MG/ML PROTEIN) AND 2 UL (0.6 M NACL, 15 % PEG 4000, 0.1 M MES PH 6.5). CRYSTAL WAS CRYOCOOLED WITH PARAFFIN OIL AS CRYOPROTECTANT. |
