2XHI
Separation-of-function mutants unravel the dual reaction mode of human 8-oxoguanine DNA glycosylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 92.669, 92.669, 210.986 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.630 - 1.550 |
R-factor | 0.23113 |
Rwork | 0.229 |
R-free | 0.26472 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yqr |
RMSD bond length | 0.025 |
RMSD bond angle | 2.291 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.400 | 1.630 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 77880 | |
<I/σ(I)> | 14.5 | 2.3 |
Completeness [%] | 99.5 | 99.6 |
Redundancy | 5.8 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |