2XE4
Structure of Oligopeptidase B from Leishmania major
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 2008-06-10 |
Detector | MARRESEARCH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 95.478, 142.776, 208.919 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 117.851 - 1.650 |
R-factor | 0.142 |
Rwork | 0.140 |
R-free | 0.17800 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.035 |
RMSD bond angle | 2.353 |
Data reduction software | d*TREK |
Data scaling software | SCALA |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.060 | 0.460 |
Number of reflections | 167772 | |
<I/σ(I)> | 8.8 | 1.7 |
Completeness [%] | 98.4 | 100 |
Redundancy | 3.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.2 | PROTEIN WAS DIALYZED INTO 50 MM TRIS, PH 8.0 AND INCUBATED WITH 10 MM ANTIPAIN FOR 30 MINS. THIS WAS MIXED IN A 1:1 RATIO WITH 25% 1,2 PROPANEDIOL, 10% GLYCEROL, 5 % PEG300 AND 0.1 M PHOSPHATE CITRATE, PH 4.2 | ||
1 | 4.2 | PROTEIN WAS DIALYZED INTO 50 MM TRIS, PH 8.0 AND INCUBATED WITH 10 MM ANTIPAIN FOR 30 MINS. THIS WAS MIXED IN A 1:1 RATIO WITH 25% 1,2 PROPANEDIOL, 10% GLYCEROL, 5 % PEG300 AND 0.1 M PHOSPHATE CITRATE, PH 4.2 |