2X7K

The crystal structure of PPIL1 in complex with cyclosporine A suggests a binding mode for SKIP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-21
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 2
Unit cell lengths	103.218, 35.701, 45.851
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.277 - 1.150
R-factor	0.1292
Rwork	0.128
R-free	0.14870
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.009
RMSD bond angle	2.205
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELXD
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.250
High resolution limit [Å]	1.150	1.150
Rmerge	0.086	0.440
Number of reflections	62933
<I/σ(I)>	8.9	2
Completeness [%]	99.8	99.4
Redundancy	6.7	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	0.1 M HEPES, PH 7.2, 0.7 M SODIUM ACETATE, 15 MM CDCL2 AND 50 MM GUANIDINE-HCL GROWN AT 4DEGC