2X0L
Crystal structure of a neuro-specific splicing variant of human histone lysine demethylase LSD1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 119.690, 181.210, 233.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.500 - 3.000 |
| R-factor | 0.21288 |
| Rwork | 0.212 |
| R-free | 0.25195 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iw5 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.571 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.4.0078) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 70.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.113 |
| Number of reflections | 49397 |
| <I/σ(I)> | 10.2 |
| Completeness [%] | 98.9 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
