2WYA
CRYSTAL STRUCTURE OF HUMAN MITOCHONDRIAL 3-HYDROXY-3-METHYLGLUTARYL- COENZYME A SYNTHASE 2 (HMGCS2)
Replaces: 2V4WExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.410, 83.514, 101.442 |
| Unit cell angles | 100.00, 108.08, 96.30 |
Refinement procedure
| Resolution | 31.358 - 1.700 |
| R-factor | 0.1616 |
| Rwork | 0.161 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p8u |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.138 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.840 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.080 | 0.380 |
| Number of reflections | 212827 | |
| <I/σ(I)> | 7.1 | 2 |
| Completeness [%] | 97.2 | 95.9 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG3350, 0.1M BISTRIS PH 6.5, 0.2M AMMONIUM SULPHATE |
