2WQB
Structure of the Tie2 kinase domain in complex with a thiazolopyrimidine inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-19 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.164, 108.791, 101.573 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.970 - 2.950 |
| R-factor | 0.19873 |
| Rwork | 0.196 |
| R-free | 0.25858 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fvr |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.494 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.970 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.120 | 0.440 |
| Number of reflections | 9451 | |
| <I/σ(I)> | 12.2 | 1.78 |
| Completeness [%] | 98.3 | 99.1 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 5 MG/ML PROTEIN, 5% (W/V) PEG6000, 5% (V/V) MPD, 100MM MOPS PH7.5, 293K |
