2W4Q
Crystal structure of human zinc-binding alcohol dehydrogenase 1 (ZADH1) in ternary complex with NADP and 18beta-glycyrrhetinic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-29 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.374, 84.110, 72.888 |
| Unit cell angles | 90.00, 109.05, 90.00 |
Refinement procedure
| Resolution | 68.840 - 2.000 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vna |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.413 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0055) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 68.900 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.100 |
| Number of reflections | 25301 |
| Completeness [%] | 99.0 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M TRIS PH 8.5; 2M (NH4)2SO4 |
