2VZE
Crystal structure of human acyl-CoA synthetase medium-chain family member 2A (L64P mutation) in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Collection date | 2008-03-18 |
| Detector | RIGAKU AREA DETECTOR |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 97.730, 97.730, 384.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.160 - 2.450 |
| R-factor | 0.196 |
| Rwork | 0.193 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b7w |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.452 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.990 | 2.710 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.010 | 0.490 |
| Number of reflections | 73402 | |
| <I/σ(I)> | 9.58 | 2 |
| Completeness [%] | 98.1 | 97.1 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M AMMONIUM ACETATE, 0.1M TRIS PH 8.5, 25% PEG 3350 |
