2VWF
Grb2 SH3C (2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2007-11-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 41.166, 41.166, 107.915 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.650 - 1.580 |
| R-factor | 0.18951 |
| Rwork | 0.188 |
| R-free | 0.21907 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vvk |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.597 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.010 | 1.000 |
| Number of reflections | 8066 | |
| <I/σ(I)> | 26.7 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 19.5 | 19.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2M TRI-AMMONIUM CITRATE, 0.1M BIS-TRIS PROPANE PH7, pH 7.0 |
