2VUW
Structure of human haspin kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.661, 78.775, 79.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.445 - 1.800 |
| R-factor | 0.1478 |
| Rwork | 0.147 |
| R-free | 0.16910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | EXPERIMENTALLY PHASED STRUCTURE FROM ANOTHER CRYSTAL OF THE SAME CONSTRUCT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.180 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 45276 | |
| <I/σ(I)> | 5.98 | 2.18 |
| Completeness [%] | 97.1 | 82.9 |
| Redundancy | 3.48 | 2.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M SPG, 60% MPG |
