2VOH
Structure of mouse A1 bound to the Bak BH3-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.239, 33.255, 58.508 |
| Unit cell angles | 90.00, 104.67, 90.00 |
Refinement procedure
| Resolution | 30.610 - 1.900 |
| R-factor | 0.185 |
| Rwork | 0.182 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vof |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.089 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.610 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.460 |
| Number of reflections | 12891 | |
| <I/σ(I)> | 19.1 | 2.9 |
| Completeness [%] | 97.1 | 95 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M SODIUM CITRATE (PH 5.6), 0.3 M (NH4)2SO4, 0.6 M LI2SO4 |
