2VKZ
Structure of the cerulenin-inhibited fungal fatty acid synthase type I multienzyme complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 231.900, 231.900, 756.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.993 - 4.000 |
| R-factor | 0.2679 |
| Rwork | 0.268 |
| R-free | 0.26750 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 4.220 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.240 | 1.000 |
| Number of reflections | 169821 | |
| <I/σ(I)> | 7 | 1.4 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
