2UZJ
Crystal structure of the mature streptococcal cysteine protease, mSpeB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.828, 60.888, 70.365 |
| Unit cell angles | 90.00, 101.14, 90.00 |
Refinement procedure
| Resolution | 21.480 - 1.550 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dki |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.341 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4I |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.480 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.090 | 0.770 |
| Number of reflections | 63795 | |
| <I/σ(I)> | 15.32 | 0.97 |
| Completeness [%] | 90.1 | 55.9 |
| Redundancy | 4.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M (NH4)2SO4, 0.1 M NA-ACETATE, PH 4.0, 20 % PEG4000. PROTEIN KONCENTRATION WAS 0.07 MM (IN 20 MM NA-ACETATE, PH 5.0). |
